isolation of pvl/acme-positive, community acquired, methicillin-resistant staphylococcus aureus (usa300) from iran

نویسندگان

amir azimian department of pathobiology and laboratory science, school of medicine, north khorasan university of medical sciences, bojnurd, iran.

seyed asghar havaei department of microbiology, school of medicine, isfahan university of medical sciences, isfahan, iran.

kiarsh ghazvini department of microbiology, school of medicine, mashhad university of medical sciences, mashhad, iran.

mahsa khosrojerdi department of pediatrics, school of medicine, semnan university of medical sciences, semnan, iran.

چکیده

introduction: methicillin-resistant staphylococcus aureus (mrsa) is responsible for an increasing number of serious hospital- and community-acquired infections. usa300 is known to be the most common cause of community-acquired infections, but recently there have been some reports on hospital-acquired infections caused by this strain. methods: totally 171 isolates of s. aureus were collected from different clinical samples in selected university hospitals in mashhad, tehran, and isfahan cities. then, they were assessed by agar screening and disk diffusion methods to determine their resistance to methicillin. the isolated mrsa strains were confirmed by detection of meca gene. the staphylococcal cassette chromosome mec (sccmec), agr, and spa typing and also detection of panton-valentine leukocidin (pvl) and arginine catabolic mobile element (acme) genes were performed on meca harboring isolates. multilocus sequence typing was performed on pvl/acme positive mrsa strains. results: we found a pvl/acme positive mrsa isolate. genetic evaluation results for this isolate produced the following profile: positive for meca, pvl, arca, and hla genes, negative for vana, sec, and tst1, and belonged to agr i, sccmec iv, sequence type 8 (st8), and spa t008. conclusion: our results suggest a finding of usa300ca-mrsa isolate in mashhad, iran. this is an uncommon finding, because usa300 is routinely found in areas other than middle east. a notable point about these isolates is that they belong to american endemic clones.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Isolation of PVL/ACME-Positive, Community Acquired, Methicillin-Resistant Staphylococcus aureus (USA300) from Iran

Introduction: Methicillin-Resistant Staphylococcus aureus (MRSA) is responsible for an increasing number of serious hospital- and community-acquired infections. USA300 is known to be the most common cause of community-acquired infections, but recently there have been some reports on hospital-acquired infections caused by this strain. Methods: Totally 171 isolates of S. aureus were collected fro...

متن کامل

Isolation of PVL/ACME-Positive, Community Acquired, Methicillin- Resistant Staphylococcus aureus (USA300) from Iran

1Department of Pathobiology and Laboratory Science, School of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran; 2Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran; 3Department of Microbiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; 4Department of Pediatrics, School of Medicine, Semnan U...

متن کامل

Community-acquired methicillin-resistant Staphylococcus aureus infection.

There was no drainage from the lesion. Two more small papules were noted on the buttocks. Physical findings were otherwise unremarkable. Wound culture was positive for methicillin-resistant Staphylococcus aureus (MRSA). Initial treatment with intravenous vancomycin was switched to intravenous clindamycin after culture results showed MRSA. The patient's symptoms resolved after 2 days of treatmen...

متن کامل

Community-Acquired Methicillin-Resistant Staphylococcus aureus, Finland

Methicillin-resistant Staphylococcus aureus (MRSA) is no longer only hospital acquired. MRSA is defined as community acquired if the MRSA-positive specimen was obtained outside hospital settings or within 2 days of hospital admission, and if it was from a person who had not been hospitalized within 2 years before the date of MRSA isolation. To estimate the proportion of community-acquired MRSA,...

متن کامل

[Community-acquired methicillin resistant Staphylococcus aureus].

Recently, methicillin-resistant Staphylococcus aureus (MRSA) has emerged as a cause of community-acquired (CA) infections among patients without established risk factors for MRSA. CA-MRSA strains mainly cause mild skin and soft tissue infections in otherwise healthy children and young adults, but can also cause severe necrotizing fasciitis and pneumonia. In contrast to nosocomial MRSA, CA-MRSA ...

متن کامل

Community-acquired Methicillin-resistant Staphylococcus aureus, Uruguay

A novel, methicillin-resistant [corrected] Staphylococcus aureus clone (Uruguay clone) with a non-multidrug-resistant phenotype caused a large outbreak, including 7 deaths, in Montevideo, Uruguay. The clone was distinct from the highly virulent community clone represented by strain MW2, although both clones carried Panton-Valentine leukocidin gene and cna gene.

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
journal of medical microbiology and infectious diseases

جلد ۲، شماره ۳، صفحات ۱۰۰-۱۰۴

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023